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ION Biosciences BAPTA AM
 
Non-fluorescent calcium (Ca²+) chelator, membrane permeable.
 
BAPTA AM is a highly selective, intracellular calcium (Ca²+) chelator that is less sensitive to pH fluctuations than EGTA and EDTA. Can be used to modulate the level of intracellular Ca²+.
Item#:
ASBAPTAAM

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ION Biosciences BCECF AM

BCECF AM is the most popular green fluorescent, intracellular pH indicator. BCECF has a pKa of ~7, and exhibits pH-dependent, dual-excitation properties (Ex/Em 430nm/535nm and 490nm/535nm) for ratiometric analysis. Ratiometry is optimal for imaging applications, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology. For HTS applications, BCECF can also be used in non-ratiometric mode using standard fluorescein excitation and emission settings.
Item#:
ASBCECFAMPN

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Brilliant Calcium Kits

High-throughput, no wash calcium (Ca²+) assay. An optimal solution for measuring intracellular Ca²+ dynamics, including effectors such as GPCRs and protein transporters.

ION Biosciences’ Brilliant Calcium (Ca²+) Assay is a total assay solution for multi-well plate-based, high-throughput measurements of dynamic changes in intracellular Ca²+. The ION Biosciences’ Brilliant Calcium Assay is useful for a wide range of effectors of intracellular Ca²+ including many G protein-coupled receptors (GPCRs), divalent cation channels, and other proteins capable of transporting Ca²+ or affecting intracellular Ca²+ dynamics. In multi-well, plate-based formats, the Brilliant Calcium Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors which can act to affect intracellular Ca²+ levels.

Brilliant Calcium Flex Kit

Our Flex kit provides all necessary reagents in a format to maximize flexibility for your assay design and customization challenges.


Contents of Brilliant Calcium Flex Kit

 

Label

Name

Volume

Containers

Storage

Reagent A

Brilliant Calcium Reagent

Dry

10

-20° C

Reagent B

DMSO

225 μL

1

4° C

Reagent C

DySolv

4 mL

1

4° C

Reagent D

10X Assay Buffer

20 mL

1

4° C

Reagent E

TRS

4 mL

1

4° C

Reagent F

Probenecid Solution

4 mL

1

4° C

  

Brilliant Calcium Express Kit  

Our Express kit is custom tailored to customer’s needs, providing only the reagents you need in the amounts you need them. Less waste, higher reproducibility, and more convenience.
  

*Research or further manufacturing use only, not for food or drug use.

Item#:
ASBRILLIANTCAL

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Brilliant Sodium Assays
 
ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na+ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na+. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
 
ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).
Item#:
ASBRTHAS

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ION Biosciences Brilliant Sodium Flex Kit
 
ION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na+ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na+. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
 
ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).
Item#:
ASBRSOFXK

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Calcein AM
 
Green fluorescent, live cell indicator.
 
Calcein AM is a membrane-permeant, non-fluorescent form of calcein that is converted to green fluorescent calcein in viable cells, resulting in uniform cytosolic fluorescence (Ex/Em 495 nm/515 nm). Calcein is well retained within the cytosol of most healthy cells with intact cell membranes.
Item#:
ASCALCEINTR

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ION Biosciences Essentials Red Calcium

Red Calcium Essentials provides the necessary reagents for multi-well plate-based, fluorescence microscopy applications and high-throughput measurements of intracellular calcium (Ca²+) mediated changes through a wide variety of plasma membrane and intracellular calcium channels and transporters. Red Calcium Essentials is also useful for investigating numerous effectors of ion channels and transporters, including G-protein coupled receptors.

Our red calcium dye, ION Calcium Red-1 (ICR-1), is a red fluorescent indicator (Ex/Em: 580/660 nm) responsive to intracellular Ca²+ dynamics. ICR-1’s spectral properties allows for multiplexing with an indicator of another color or GFP-tagged cells.

When following the recommended protocol, Red Calcium Essentials provides enough reagents to make 100 mL of working solution, enough for ten 96-well plates. The actual number of assays will vary according to optimal dye concentrations for your application.
Item#:
ASESSREDCAL

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ION Biosciences Fluo-2 AM

Green fluorescent, calcium (Ca²+) indicator, membrane permeable. Higher Ca²+ affinity than Fluo-4.
 
Fluo-2 is a green fluorescent, intracellular calcium (Ca²+) indicator. Fluo-2 has higher Ca²+ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²+ concentrations.
Item#:
ASFLUO2AM

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ION Biosciences Fluo-2 K+ Salt

Green fluorescent, calcium (Ca²+) indicator, membrane impermeable. Higher Ca²+ affinity than Fluo-4.

Fluo-2 is a green fluorescent, calcium (Ca²+) indicator. Fluo-2 has higher Ca²+ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²+ concentrations.

Fluo-2 K+ salt is a membrane impermeable form of Fluo-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

Item#:
ASFLUO2AMST

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ION Biosciences Fluo-3 AM

Green fluorescent, calcium (Ca²+) indicator, membrane permeable.
 
Fluo-3 is a green fluorescent, intracellular calcium (Ca²+) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications. Is preferred over Fluo-4 for some cell types.
Item#:
ASFLUO3AM

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ION Biosciences Fluo-3 K+ Salt

Green fluorescent, calcium (Ca²+) indicator, membrane impermeable
 
Fluo-3 is a green fluorescent, intracellular calcium (Ca²+) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications.Is preferred over Fluo-4 for some cell types.
 
Fluo-3 K+ salt is a membrane impermeable form of Fluo-3 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFLUO3AMST

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ION Biosciences Fluo-4 AM

Green fluorescent, calcium (Ca²+) indicator, membrane permeable.
 
Fluo-4 is the most popular green fluorescent, intracellular calcium (Ca²+) indicator. Proven and trusted for decades to measure intracellular Ca²+ flux in high throughput screening and fluorescence microscopy applications.
Item#:
ASFLUO4AM
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